Abstract
Transforming growth factor beta (TGF-beta) was tested for its ability to stimulate a chemotactic response in Stage 24 embryonic chick limb bud mesenchymal cells and muscle-derived fibroblasts. TGF-beta stimulated dose-dependent chemotaxis in both cell populations. Maximal chemotaxis was achieved with a concentration of 5 ng/ml for limb bud cells and as low as 15 pg/ml for muscle-derived fibroblasts. TGF-beta was not chemokinetic at these levels. Several other proteins found in bone, namely fibronectin, type I collagen, and osteonectin, were not chemotactic. However, both Bone Gla-protein and basic-FGF were found to be chemotactic but less effective than TGF-beta. Comparison with extracts of adult bone indicates that while TGF-beta is a potent chemoattractant, it does not account for all the chemotactic activity found in adult bone.
Keywords
ChemotaxisLimb budOsteonectinTransforming growth factorMesenchymal stem cellCell biologyFibronectinEmbryonic stem cellFibroblast growth factorBiologyChemistryInternal medicineEndocrinologyEmbryoExtracellular matrixBiochemistryMedicineAlkaline phosphatase
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Publication Info
- Year
- 1988
- Type
- article
- Volume
- 18
- Issue
- 1
- Pages
- 1-7
- Citations
- 51
- Access
- Closed
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Cite This
Paul A. Lucas,
Arnold I. Caplan
(1988).
Chemotactic response of embryonic limb bud mesenchymal cells and muscle-derived fibroblasts to transforming growth factor-β.
Connective Tissue Research
, 18
(1)
, 1-7.
https://doi.org/10.3109/03008208809019068
Identifiers
- DOI
- 10.3109/03008208809019068