Abstract
A major goal of proteomics is to develop methods that enable the systematic characterization of every protein within the cell or particular subcellular proteome using a single analytical platform. Although the equivalent has already been achieved in genomics, reaching this goal in proteomics represents a much greater challenge due to the wide dynamic range of protein expression, numerous post-translational modifications and remarkable physicochemical heterogeneity of proteins. A major analytical challenge has involved developing more effective means for proteome-scale investigations of membrane proteins, whose solubility differs drastically from that of cytoplasmic proteins. Fortunately, rapid progress has increased the ability to characterize this critically important class of proteins on a scale analogous to that of aqueous soluble proteins.
Keywords
ProteomeProteomicsComputational biologyQuantitative proteomicsMembrane proteinHuman proteome projectBiologyChemistryBioinformaticsBiochemistryMembraneGene
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Publication Info
- Year
- 2004
- Type
- review
- Volume
- 1
- Issue
- 2
- Pages
- 153-163
- Citations
- 38
- Access
- Closed
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Cite This
Josip Blonder,
Thomas P. Conrads,
Timothy D. Veenstra
(2004).
Characterization and quantitation of membrane proteomes using multidimensional MS-based proteomic technologies.
Expert Review of Proteomics
, 1
(2)
, 153-163.
https://doi.org/10.1586/14789450.1.2.153
Identifiers
- DOI
- 10.1586/14789450.1.2.153