Abstract
Abstract Previously we have shown that autoimmune diabetes, induced in rats by a protocol of adult thymectomy and split-dose gamma irradiation, can be prevented by the transfer of a subset of CD4+ T cells with a memory phenotype (CD45RC−), as well as by CD4+CD8− thymocytes, from syngeneic donors. Further studies now reveal that in the thymus the regulatory cells are observed in the CD25+ subset of CD4+CD8− cells, whereas transfer of the corresponding CD25− thymocyte subset leads to acceleration of disease onset in prediabetic recipients. However, in the periphery, not all regulatory T cells were found to be CD25+. In thoracic duct lymph, cells that could prevent diabetes were found in both CD25− and CD25+ subsets of CD4+CD45RC− cells. Further, CD25− regulatory T cells were also present within the CD4+CD45RC− cell subset from spleen and lymph nodes, but were effective in preventing diabetes only after the removal of CD25− recent thymic emigrants. Phenotypic analysis of human thymocytes showed the presence of CD25+ cells in the same proportions as in rat thymus. The possible developmental relationship between CD25+ and CD25− regulatory T cells is discussed.
Keywords
IL-2 receptorCD8BiologyImmunologyThymocyteSpleenAdoptive cell transferEndocrinologyT cellAntigenImmune system
MeSH Terms
Adoptive TransferAnimalsBiomarkersCD4-Positive T-LymphocytesCD8 AntigensCell DifferentiationCell MovementCell SeparationDiabetes MellitusType 1FemaleImmunologic MemoryLymph NodesLymphocyte DepletionRadiation ChimeraRatsReceptorsInterleukin-2SpleenT-Lymphocyte SubsetsThoracic DuctThymectomyThymus Gland
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Publication Info
- Year
- 2000
- Type
- article
- Volume
- 165
- Issue
- 6
- Pages
- 3105-3110
- Citations
- 311
- Access
- Closed
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Cite This
Leigh A. Stephens,
Don Mason
(2000).
CD25 Is a Marker for CD4+ Thymocytes That Prevent Autoimmune Diabetes in Rats, But Peripheral T Cells with This Function Are Found in Both CD25+ and CD25− Subpopulations.
The Journal of Immunology
, 165
(6)
, 3105-3110.
https://doi.org/10.4049/jimmunol.165.6.3105
Identifiers
- DOI
- 10.4049/jimmunol.165.6.3105
- PMID
- 10975823