Abstract
A procedure is described to utilize blue dextran-Sepharose as an affinity chromatographic column specific for the super-secondary structure called the dinucleotide fold, which forms the binding sites for substrates and effectors on a wide range of proteins. The procedure can be used to identify proteins, either purified or in crude cellular extracts, that possess the dinucleotide fold and to significantly improve the purification procedures for those proteins that possess the fold.
Keywords
SepharoseAffinity chromatographyChemistryChromatographyDextranBiochemistryEnzyme
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Publication Info
- Year
- 1975
- Type
- article
- Volume
- 72
- Issue
- 2
- Pages
- 669-672
- Citations
- 423
- Access
- Closed
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Cite This
Sioe Thung Thompson,
Kathleen Heelan Cass,
Earle Stellwagen
(1975).
Blue dextran-sepharose: an affinity column for the dinucleotide fold in proteins..
Proceedings of the National Academy of Sciences
, 72
(2)
, 669-672.
https://doi.org/10.1073/pnas.72.2.669
Identifiers
- DOI
- 10.1073/pnas.72.2.669