Abstract
Abstract An automated colorimetric procedure is described for the determination of serum and urine uric acid with improved specificity. Hydrogen peroxide, formed when uric acid is oxidized by uricase, oxidatively couples with 3-methyl-2-benzothiazolinone hydrazone and N,N-dimethylaniline, in the presence of peroxidase, to produce a stable, sensitive indamine dye. The method is reproducible, and the results correlate well with those obtained by a manual spectrophotometric uricase procedure. Also, the automated procedure is resistant to many added reducing substances that cause positive interferences in alkaline phosphotungstate methods.
Keywords
Uric acidChemistryHydrogen peroxidePeroxidaseHydrazoneUrate oxidaseChromatographyDimethylanilineUrinePeroxideColorimetryBiochemistryNuclear chemistryOrganic chemistryEnzyme
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Publication Info
- Year
- 1971
- Type
- article
- Volume
- 17
- Issue
- 12
- Pages
- 1154-1159
- Citations
- 155
- Access
- Closed
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Cite This
Nathan Gochman,
Joan M Schmitz
(1971).
Automated Determination of Uric Acid, with Use of a Uricase—Peroxidase System.
Clinical Chemistry
, 17
(12)
, 1154-1159.
https://doi.org/10.1093/clinchem/17.12.1154
Identifiers
- DOI
- 10.1093/clinchem/17.12.1154