Abstract
Abstract The anti-My-10 mouse monoclonal antibody was raised against the immature human myeloid cell line KG-1a and was selected for nonreactivity with mature human granulocytes. Anti-My-10 immunoprecipitated a KG-1a cell surface protein with an apparent Mr of approximately 115 kD. We describe the binding of this antibody to human hematopoietic cell types and show that My-10 is expressed specifically on immature normal human marrow cells, including hematopoietic progenitor cells. My-10 is also expressed by leukemic marrow cells from a subpopulation of patients. Thus, this antibody allows the identification and purification of hematopoietic progenitor cells from normal human marrow and the subclassification of leukemia.
Keywords
HaematopoiesisMonoclonal antibodyProgenitor cellAntigenBiologyMolecular biologyMyeloidAntibodyMyeloid leukemiaStem cellImmunologyCell biology
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Publication Info
- Year
- 1984
- Type
- article
- Volume
- 133
- Issue
- 1
- Pages
- 157-165
- Citations
- 1180
- Access
- Closed
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Cite This
CI Civin,
Lewis C. Strauss,
Charlotte Brovall
et al.
(1984).
Antigenic analysis of hematopoiesis. III. A hematopoietic progenitor cell surface antigen defined by a monoclonal antibody raised against KG-1a cells..
The Journal of Immunology
, 133
(1)
, 157-165.
https://doi.org/10.4049/jimmunol.133.1.157
Identifiers
- DOI
- 10.4049/jimmunol.133.1.157