Amplification of the structurally and functionally altered epidermal growth factor receptor gene (c-erbB) in human brain tumors.

Abstract

By using Southern blot analysis, we found that in two cases of human glioblastoma multiforme, cells carried amplified c-erbB genes which bore short deletion mutations within the ligand-binding domain of the epidermal growth factor (EGF) receptor. The products of these mutated c-erbB genes were about 30 kilodalton (kDa) smaller than the normal 170-kDa EGF receptor, and the tumor cell membrane fractions containing the 140-kDa abnormal EGF receptor showed a significant elevation of tyrosine kinase activity without its ligand. In view of the similarity to the activated viral and cellular erbB genes in the avian system, these mutated and overexpressed EGF receptors might play a role in the onset or development of human glioblastoma cells.

Keywords

BiologyErbBEpidermal growth factorEpidermal growth factor receptorMolecular biologyReceptorReceptor tyrosine kinaseTyrosine kinaseGeneGrowth factor receptorCell surface receptorCancer researchCell biologyGenetics

Affiliated Institutions

The University of Tokyo JP

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Publication Info

Year: 1988
Type: article
Volume: 8
Issue: 4
Pages: 1816-1820
Citations: 214
Access: Closed

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APA Style

                            
                                
                                    H. Yamazaki, 
                                
                                    Yoshinori Fukui, 
                                
                                    Yoshito Ueyama
                                
                                et al.
                            
                            (1988). 
                            Amplification of the structurally and functionally altered epidermal growth factor receptor gene (c-erbB) in human brain tumors.. 
                            Molecular and Cellular Biology
                            , 8
                            (4)
                            , 1816-1820.
                            https://doi.org/10.1128/mcb.8.4.1816
                        

Identifiers

DOI: 10.1128/mcb.8.4.1816