Abstract

µManager is an open-source, cross-platform desktop application, to control a wide variety of motorized microscopes, scientific cameras, stages, illuminators, and other microscope accessories. Since its inception in 2005, µManager has grown to support a wide range of microscopy hardware and is now used by thousands of researchers around the world. The application provides a mature graphical user interface and offers open programming interfaces to facilitate plugins and scripts. Here, we present a guide to using some of the recently added advanced µManager features, including hardware synchronization, simultaneous use of multiple cameras, projection of patterned light onto a specimen, live slide mapping, imaging with multi-well plates, particle localization and tracking, and high-speed imaging.

Keywords

MicroscopeComputer scienceScripting languageSoftwareComputer graphics (images)Graphical user interfaceProjection (relational algebra)Interface (matter)Tracking (education)Computer visionComputer hardwareOperating systemOptics

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Publication Info

Year
2014
Type
article
Volume
1
Issue
2
Pages
1-1
Citations
2052
Access
Closed

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Arthur Edelstein, Mark A. Tsuchida, Nenad Amodaj et al. (2014). Advanced methods of microscope control using μManager software. Journal of Biological Methods , 1 (2) , 1-1. https://doi.org/10.14440/jbm.2014.36

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DOI
10.14440/jbm.2014.36