Adipose tissue-derived microRNAs as epigenetic modulators of type 2 diabetes

Abstract

Abstract Background White adipose tissue (WAT) dysfunction including an aberrant expression of miRNAs is strongly associated with the risk of developing type 2 diabetes (T2D), with limited evidence linking early changes in the WAT-derived miRNAs and T2D. The present study aims to identify early miRNome changes prognostic for T2D in mice and humans. Methods Gonadal (g) WAT of diabetes-resistant and diabetes-prone mice were subjected to multi-omics analyses (transcriptome, miRNome, methylome, proteome). Metabolic phenotypes linked with T2D were correlated with adipose tissue miRNA expression and DNA methylation from 14 monozygotic twin pairs discordant for T2D. Plasma miRNA levels from females at high risk of developing T2D (TÜF study) were included. Results Adipose tissue of the diabetes-susceptible mice was less insulin sensitive with ~ 200 differentially expressed mature miRNAs compared to diabetes-resistant mice. Integrative analysis of miRNome-transcriptome-proteome identified 227 proteins involved in amino acid metabolism, inflammation, signalling pathways, and insulin resistance. More than 20 differentially expressed miRNAs are located in the imprinted region Dlk1-Gtl2 and Mest (miR-335) potentially regulated by DNA methylation. Imprinted miRNAs also exhibited similar alterations in adipose tissue from monozygotic twin pairs discordant for T2D, with miR-335 expression altered only in females. Moreover, plasma levels of miR-335-5p were negatively correlated with fasting blood glucose in females at high risk of developing T2D. Conclusions Early alterations of WAT-derived miRNAs such as miR-335-5p could contribute to systemic metabolic changes associated with the risk of developing T2D.

Affiliated Institutions

• Heinrich Heine University Düsseldorf DE
• Leibniz Institute for Analytical Sciences - ISAS DE
• University of Tübingen DE
• Universität Ulm DE
• University of Potsdam DE
• University Hospital Ulm DE
• Scania (Sweden) SE
• Copenhagen University Hospital DK
• German Center for Diabetes Research DE
• Steno Diabetes Centers DK
• University Children's Hospital Tübingen DE
• German Institute of Human Nutrition DE
• Lund University SE
• Deutsches Diabetes-Zentrum e.V. DE

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