Abstract
The signal transduction mechanisms of interferons (IFNs) remain unclear partly because no effect of IFN has been reproducible in a cell-free system. IFN-α rapidly induces the transcription of a set of early response genes, and a multicomponent transcriptional activator, interferon stimulated gene factor 3 (ISGF3), is activated within minutes after binding of IFN-α to its receptor. A system was developed in which IFN-α activated ISGF3 in homogenates of HeLa cells. Subcellular fractionation revealed that incubation of a plasma membrane-enriched fraction with IFN-α was sufficient to activate the regulatory subcomponent of ISGF3.
Keywords
Activator (genetics)HeLaAlpha interferonCell-free systemCell biologyTranscription factorTranscription (linguistics)Interferon regulatory factorsInterferonAlpha (finance)BiologySignal transductionCellMolecular biologyIncubationChemistryGeneBiochemistryIn vitroImmunology
Affiliated Institutions
Related Publications
Requirement for STAT1 in LPS-induced gene expression in macrophages
Abstract This study examines the role of the signal transducer and activator of transcription 1 (STAT1) in induction of lipopolysaccharide (LPS)-stimulated gene expression both ...
HOW CELLS RESPOND TO INTERFERONS
Interferons play key roles in mediating antiviral and antigrowth responses and in modulating immune response. The main signaling pathways are rapid and direct. They involve tyro...
Toll-Like Receptor Signaling and Regulation of Cytokine Gene Expression in the Immune System
Dendritic cells, macrophages, and granulocytes are derived from hematopoietic stem cells and provide a first line of defense against infectious pathogens. Toll-like receptors (T...
Impaired type I interferon activity and inflammatory responses in severe COVID-19 patients
Interferons interfere with lung repair Interferons (IFNs) are central to antiviral immunity. Viral recognition elicits IFN production, which in turn triggers the transcription o...
The Double-Stranded RNA-Dependent Protein Kinase PKR: Structure and Function
This review describes the structure and function of the interferon (IFN)-inducible, double-stranded RNA-activated protein kinase PKR. This protein kinase has been studied extens...
Publication Info
- Year
- 1992
- Type
- article
- Volume
- 257
- Issue
- 5071
- Pages
- 813-815
- Citations
- 89
- Access
- Closed
External Links
Social Impact
Altmetric
PlumX Metrics
Social media, news, blog, policy document mentions
Citation Metrics
89
OpenAlex
Cite This
Michael David,
Andrew C. Larner
(1992).
Activation of Transcription Factors by Interferon-Alpha in a Cell-Free System.
Science
, 257
(5071)
, 813-815.
https://doi.org/10.1126/science.1496402
Identifiers
- DOI
- 10.1126/science.1496402