Abstract
A mechanical fractionator was developed to produce electrophorograms by extrusion of polyacrylamide gels through a narrow orifice in a continuous, sequential stream. The system permits separation of uniform fractions free of zone distortion. An electrophorogram of radioactive type-2 adenovirus proteins so fractionated gave a pattern in excellent agreement with the pattern obtained by laborious manual sectioning and in agreement with the pattern obtained on a replicate gel stained with Coomassie brilliant blue R250. The adenovirus particle yielded about ten resolvable protein components in unequal amounts. Like picornaviruses, these icosahedral animal viruses have multiple protein components in the viral coat.
Keywords
Coomassie Brilliant BlueAcrylamideFractionationPolyacrylamide gel electrophoresisChromatographyExtrusionPolyacrylamideChemistryAgaroseMolecular biologyBiologyMaterials scienceBiochemistryStainingComposite material
Affiliated Institutions
Related Publications
α‐Synuclein Immunoisolation of Glial Inclusions from Multiple System Atrophy Brain Tissue Reveals Multiprotein Components
Immunohistochemical studies have shown that oligodendroglial inclusions in multiple system atrophy contain α-synuclein, a synaptic protein also found in Lewy bodies in Parkinson...
A point‐charge force field for molecular mechanics simulations of proteins based on condensed‐phase quantum mechanical calculations
Abstract Molecular mechanics models have been applied extensively to study the dynamics of proteins and nucleic acids. Here we report the development of a third‐generation point...
Publication Info
- Year
- 1966
- Type
- article
- Volume
- 151
- Issue
- 3713
- Pages
- 988-990
- Citations
- 566
- Access
- Closed
External Links
Social Impact
Altmetric
PlumX Metrics
Social media, news, blog, policy document mentions
Citation Metrics
566
OpenAlex
Cite This
Jacob V. Maizel
(1966).
Acrylamide-Gel Electrophorograms by Mechanical Fractionation: Radioactive Adenovirus Proteins.
Science
, 151
(3713)
, 988-990.
https://doi.org/10.1126/science.151.3713.988
Identifiers
- DOI
- 10.1126/science.151.3713.988