Abstract
We report that a subpopulation (10%) of the Mac-1 (CD1 1b/CD18) molecules on activated neutrophils mediates adhesion to ICAM-1 and fibrinogen. We describe a novel mAb (CBRM1/5) that binds to an activation-specific neoepitope on a subset of Mac-1 molecules on neutrophils and monocytes after stimulation with chemoattractants or phorobol esters but does not recognize Mac-1 on resting myeloid cells. CBRM1/5 immunoprecipitates a subpopulation of Mac-1 molecules from detergent lysates of neutrophils, binds to immunoaffinity-purified Mac-1, and localizes to the I domain on the alpha chain of Mac-1. Because CBRM1/5 recognizes a fraction of Mac-1 on activated neutrophils, but still blocks Mac-1-dependent adhesion to fibrinogen and ICAM-1, we suggest that only a small subset of Mac-1 molecules is competent to mediate adhesion.
Keywords
CD18BiologyCell adhesion moleculeIntegrin alpha MCell biologyCell adhesionIntegrinChemotaxisFibrinogenMolecular biologyReceptorBiochemistryFlow cytometryCell
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Publication Info
- Year
- 1993
- Type
- article
- Volume
- 120
- Issue
- 2
- Pages
- 545-556
- Citations
- 513
- Access
- Closed
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Cite This
Michael Diamond,
Timothy A. Springer
(1993).
A subpopulation of Mac-1 (CD11b/CD18) molecules mediates neutrophil adhesion to ICAM-1 and fibrinogen..
The Journal of Cell Biology
, 120
(2)
, 545-556.
https://doi.org/10.1083/jcb.120.2.545
Identifiers
- DOI
- 10.1083/jcb.120.2.545