Abstract
Posttranscriptional gene silencing (PTGS) is a nucleotide sequence–specific defense mechanism that can target both cellular and viral mRNAs. Here, three types of transgene-induced PTGS and one example of virus-induced PTGS were analyzed in plants. In each case, antisense RNA complementary to the targeted mRNA was detected. These RNA molecules were of a uniform length, estimated at 25 nucleotides, and their accumulation required either transgene sense transcription or RNA virus replication. Thus, the 25-nucleotide antisense RNA is likely synthesized from an RNA template and may represent the specificity determinant of PTGS.
Keywords
RNA silencingRNABiologyAntisense RNASense (electronics)Gene silencingTranscription (linguistics)GeneRNA interferenceRNA-induced silencing complexTransgeneRNA-induced transcriptional silencingGeneticsTrans-acting siRNANucleotideSmall RNAMolecular biologyChemistry
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Publication Info
- Year
- 1999
- Type
- letter
- Volume
- 286
- Issue
- 5441
- Pages
- 950-952
- Citations
- 3003
- Access
- Closed
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Cite This
Andrew J. Hamilton,
David C. Baulcombe
(1999).
A Species of Small Antisense RNA in Posttranscriptional Gene Silencing in Plants.
Science
, 286
(5441)
, 950-952.
https://doi.org/10.1126/science.286.5441.950
Identifiers
- DOI
- 10.1126/science.286.5441.950