Abstract

The lipophilic CdSe quantum dot (QD) coated with trioctylphosphine oxide (TOPOQD) can be extracted from chloroform into water upon interaction with macrocyclic glycocluster amphiphile 1. The QD-conjugated and highly fluorescent sugar ball of a size of 15 nm (TOPOQD1) thus solubilized in water readily invades Hela cells via endocytosis. The endocytic activity of TOPOQD1 (15 nm), in light of those of the micellar homoaggregate of 1 (5 nm) and the virus-like 1-DNA conjugate (50 nm) as references, reveals a dramatic size effect (50 > 15 > 5) in the subviral size region. The optimal size at approximately 50 nm indicates that size complementarity which governs molecular recognition in small host-guest systems also plays key roles in the encapsulation of nanometric guest particles by the endocytic vesicles (</=100 nm) as a macrobiomolecular host. The work thus suggests an utmost importance of size control at the viral size when designing molecular (gene, drug, probe, etc.) delivery machines.

Keywords

ChemistryConjugated systemEndocytosisQuantum dotNanotechnologyBiophysicsAllophycocyaninAmphiphileEndocytic cycleBiochemistryOrganic chemistryCopolymer

MeSH Terms

EndocytosisGene Transfer TechniquesHeLa CellsHumansMicroscopyElectronMicroscopyFluorescenceNanotechnologyOligosaccharidesParticle SizeQuantum DotsViruses

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Publication Info

Year
2004
Type
article
Volume
126
Issue
21
Pages
6520-6521
Citations
527
Access
Closed

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Cite This

Fumio Osaki, Takuya Kanamori, Shinsuke Sando et al. (2004). A Quantum Dot Conjugated Sugar Ball and Its Cellular Uptake. On the Size Effects of Endocytosis in the Subviral Region. Journal of the American Chemical Society , 126 (21) , 6520-6521. https://doi.org/10.1021/ja048792a

Identifiers

DOI
10.1021/ja048792a
PMID
15161257

Data Quality

Data completeness: 81%