A p53-Dependent Mouse Spindle Checkpoint

Shawn M. Cross; Carissa A. Sanchez; Catherine Morgan; Melana K. Schimke; Stig Ramel; Rejean L. Idzerda; Wendy H. Raskind; Brian J. Reid

doi:10.1126/science.7871434

Abstract

Cell cycle checkpoints enhance genetic fidelity by causing arrest at specific stages of the cell cycle when previous events have not been completed. The tumor suppressor p53 has been implicated in a G1 checkpoint. To investigate whether p53 also participates in a mitotic checkpoint, cultured fibroblasts from p53-deficient mouse embryos were exposed to spindle inhibitors. The fibroblasts underwent multiple rounds of DNA synthesis without completing chromosome segregation, thus forming tetraploid and octaploid cells. Deficiency of p53 was also associated with the development of tetraploidy in vivo. These results suggest that murine p53 is a component of a spindle checkpoint that ensures the maintenance of diploidy.

Keywords

Spindle checkpointCell cycle checkpointG2-M DNA damage checkpointBiologyCell biologyMitosisCell cycleChromosome segregationCHEK1ChromosomeSuppressorSpindle apparatusCancer researchGeneticsCell divisionCellCancerGene

MeSH Terms

AnimalsCell CycleCellsCulturedDNADemecolcineDiploidyFemaleGenesp53MaleMiceMitosisNocodazolePloidiesSpindle ApparatusTumor Suppressor Protein p53

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Publication Info

Year: 1995
Type: article
Volume: 267
Issue: 5202
Pages: 1353-1356
Citations: 671
Access: Closed

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APA Style

                            
                                
                                    Shawn M. Cross, 
                                
                                    Carissa A. Sanchez, 
                                
                                    Catherine Morgan
                                
                                et al.
                            
                            (1995). 
                            A p53-Dependent Mouse Spindle Checkpoint. 
                            Science
                            , 267
                            (5202)
                            , 1353-1356.
                            https://doi.org/10.1126/science.7871434
                        

Identifiers

DOI: 10.1126/science.7871434
PMID: 7871434

Data Quality

Data completeness: 81%