Keywords
BiologyRestriction enzymeFragment (logic)Shotgun sequencingCloning vectorGeneticsCloning (programming)Multiple cloning siteComputational biologyDNARestriction siteRestriction fragmentIn vitro recombinationEcoRIMolecular biologyMolecular cloningDNA sequencingVector (molecular biology)Recombinant DNAAlgorithmComplementary DNAGeneComputer science
Affiliated Institutions
Related Publications
Genome sequencing on both strands: the Janus strategy
The design of large scale DNA sequencing projects such as genome analysis demands a new approach to sequencing strategy, since neither a purely random nor a purely directed meth...
Ligation-independent cloning of PCR products (LIC-PCR)
A new procedure has been developed for the efficient cloning of complex PCR mixtures, resulting in libraries exclusively consisting of recombinant clones. Recombinants are gener...
Filamentous coliphage M13 as a cloning vehicle: insertion of a HindII fragment of the lac regulatory region in M13 replicative form in vitro.
A HindII restriction fragment comprising the Escherichia coli lac regulatory region and the genetic information for the alpha peptide of beta-galactosidase (beta-D-galactosidega...
Yeast/<i>E. coli</i> shuttle vectors with multiple unique restriction sites
Abstract Two yeast/ E. coli shuttle vectors have been constructed. The two vectors, YEp351 and YEp352, have the following properties: (1) they can replicate autonomuosly in Sacc...
Directed mutagenesis of DNA cloned in filamentous phage: influence of hemimethylated GATC sites on marker recovery from restriction fragments
Gapped duplex DNA molecules of recombinant genomes of filamentous phage are constructed in vitro. Denatured restriction fragments covering (part of) the precisely constructed ga...
Publication Info
- Year
- 1982
- Type
- article
- Volume
- 19
- Issue
- 3
- Pages
- 269-276
- Citations
- 2941
- Access
- Closed
External Links
Social Impact
Altmetric
PlumX Metrics
Social media, news, blog, policy document mentions
Citation Metrics
2941
OpenAlex
Cite This
Joachim Messing,
Jeffrey Vieira
(1982).
A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.
Gene
, 19
(3)
, 269-276.
https://doi.org/10.1016/0378-1119(82)90016-6
Identifiers
- DOI
- 10.1016/0378-1119(82)90016-6