A METTL3–METTL14 complex mediates mammalian nuclear RNA N6-adenosine methylation

Abstract

N6-methyladenosine (m6A) is the most prevalent and reversible internal modification in mammalian messenger and non-coding RNAs. We report here that human METTL14 catalyzes m6A RNA methylation. Together with METTL3, the only previously known m6A methyltransferase, these two proteins form a stable heterodimer core complex of METTL3-14 that functions in cellular m6A deposition on mammalian nuclear RNAs. WTAP, a mammalian splicing factor, can interact with this complex and affect this methylation.

Keywords

RNA methylationMethylationMethyltransferaseRNA splicingRNACell biologyN6-MethyladenosineBiologyNon-coding RNAFive-prime capBiochemistryChemistryGene

MeSH Terms

AdenosineAnimalsBase SequenceBinding SitesCell NucleusEnzyme StabilityHeLa CellsHumansMethylationMethyltransferasesModelsMolecularMultienzyme ComplexesRNA

Affiliated Institutions

University of Chicago US

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Publication Info

Year: 2013
Type: article
Volume: 10
Issue: 2
Pages: 93-95
Citations: 3388
Access: Closed

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APA Style

                            
                                
                                    Jianzhao Liu, 
                                
                                    Yanan Yue, 
                                
                                    Dali Han
                                
                                et al.
                            
                            (2013). 
                            A METTL3–METTL14 complex mediates mammalian nuclear RNA N6-adenosine methylation. 
                            Nature Chemical Biology
                            , 10
                            (2)
                            , 93-95.
                            https://doi.org/10.1038/nchembio.1432
                        

Identifiers

DOI: 10.1038/nchembio.1432
PMID: 24316715
PMCID: PMC3911877

Data Quality

Data completeness: 86%