A Map of the Interactome Network of the Metazoan <i>C. elegans</i>

Abstract

To initiate studies on how protein-protein interaction (or “interactome”) networks relate to multicellular functions, we have mapped a large fraction of the Caenorhabditis elegans interactome network. Starting with a subset of metazoan-specific proteins, more than 4000 interactions were identified from high-throughput, yeast two-hybrid (HT=Y2H) screens. Independent coaffinity purification assays experimentally validated the overall quality of this Y2H data set. Together with already described Y2H interactions and interologs predicted in silico , the current version of the Worm Interactome (WI5) map contains ∼5500 interactions. Topological and biological features of this interactome network, as well as its integration with phenome and transcriptome data sets, lead to numerous biological hypotheses.

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Affiliated Institutions

• Dana-Farber Cancer Institute US
• Indiana University Bloomington US
• Harvard University US
• University of Utah US
• New York University US
• University of Namur BE
• Institut de Génétique Moléculaire de Montpellier FR
• Yale University US
• Baylor College of Medicine US

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