A 718-kb DNA Sequence of the Escherichia coli K-12 Genome Corresponding to the 12.7-28.0 min Region on the Linkage Map

Abstract

The 718,122 base pair sequence of the Escherichia coli K-12 genome corresponding to the region from 12.7 to 28.0 minutes on the genetic map is described. This region contains at least 681 potential open reading frames, of which 277 (41%) have been previously identified, 147 (22%) are homologous to other known genes, 139 (20%) are identical or similar to the hypothetical genes registered in databases, and the remaining 118 (17%) do not show a significant similarity to any other gene. In this region, we assigned a cluster of cit genes encoding multienzyme citrate lyase, two clusters of fimbrial genes and a set of lysogenic phage genes encoding integrase, excisionase and repressor in the e14 genetic element. In addition, a new valine tRNA gene, designated valZ, and a family of long directly repeated sequences, LDR-A, -B and -C, were found.

Keywords

Affiliated Institutions

• Kobe University JP
• Kyushu University JP
• Kyoto University JP
• National Institute for Basic Biology JP
• Nara Institute of Science and Technology JP
• Teikyo University JP
• Tokyo University of Science JP
• Hyogo Medical University JP
• The University of Tokyo JP
• The University of Osaka JP
• University of Electro-Communications JP
• Nagoya University JP

Related Publications