Abstract
1,25-Dihydroxyvitamin D3 [1,25-(OH)2D3], but not 24,25-(OH)2D3 stimulates the alkaline phosphatase activity of cultured human bone cell populations. The stimulatory effect of the sterol was dose dependent (10(-10)-10(-7) M), evident by 24 h, and observed over a range of cell densities. Analysis of the radiolabeled collagens synthesised by human bone cell cultures indicated the synthesis of predominantly type I collagen. In the presence of 1,25-(OH)2D3, but not 24,25-(OH)2D3, there was a dose-dependent (10(-11)-10(-9) M) increase in radiolabeled proline incorporation into collagenase-digestible protein and in the amount of collagen synthesized, expressed as a percentage of the total protein synthesis. The effect of 1,25-(OH)2D3 was observed over a range of cell densities and appeared to be specific for the synthesis of type I collagen. The stimulatory effect of 1,25-(OH)2D3 on alkaline phosphatase activity and the increase in proline incorporation into collagenase-digestible protein were accompanied by a dose-dependent (5 X 10(-11) to 5 X 10(-8) M) inhibition of bone cell proliferation. These findings suggest that 1,25-(OH)2D3 is an important modulator of the growth and differentiation of human bone cells in vitro. They are also consistent with the possibility that 1,25-(OH)2D3 has direct effects on bone formation in vivo.
Keywords
Alkaline phosphataseCollagenaseEndocrinologyInternal medicineIn vitroCell growthChemistryIn vivoBone cellType I collagenProlineCell cultureBiologyBiochemistryEnzymeMedicineAmino acid
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Publication Info
- Year
- 1986
- Type
- article
- Volume
- 119
- Issue
- 4
- Pages
- 1776-1785
- Citations
- 279
- Access
- Closed
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Cite This
J.N. Beresford,
J.A. Gallagher,
R.G.G. Russell
(1986).
1,25-Dihydroxyvitamin D<sub>3</sub>and Human Bone-Derived Cells in Vitro: Effects on Alkaline Phosphatase, Type I Collagen and Proliferation*.
Endocrinology
, 119
(4)
, 1776-1785.
https://doi.org/10.1210/endo-119-4-1776
Identifiers
- DOI
- 10.1210/endo-119-4-1776