Cooperative Institute for Research in Environmental Sciences

QIIME allows analysis of high-throughput community sequencing data

Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample

The ongoing revolution in high-throughput sequencing continues to democratize the ability of small groups of investigators to map the microbial component of the biosphere. In pa...

Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms

Abstract DNA sequencing continues to decrease in cost with the Illumina HiSeq2000 generating up to 600 Gb of paired-end 100 base reads in a ten-day run. Here we present a protoc...

Pyrosequencing-Based Assessment of Soil pH as a Predictor of Soil Bacterial Community Structure at the Continental Scale

ABSTRACT Soils harbor enormously diverse bacterial populations, and soil bacterial communities can vary greatly in composition across space. However, our understanding of the sp...

The Twentieth Century Reanalysis Project

Abstract The Twentieth Century Reanalysis (20CR) project is an international effort to produce a comprehensive global atmospheric circulation dataset spanning the twentieth cent...

Bacterial Community Variation in Human Body Habitats Across Space and Time

Growing on You The human gut and skin harbor diverse microbial communities that are known to vary strikingly among individuals. Here, Costello et al. (p. 1694 , published online...

Cross-biome metagenomic analyses of soil microbial communities and their functional attributes

For centuries ecologists have studied how the diversity and functional traits of plant and animal communities vary across biomes. In contrast, we have only just begun exploring ...

Unlocking the potential of metagenomics through replicated experimental design

Metagenomics holds enormous promise for discovering novel enzymes and organisms that are biomarkers or drivers of processes relevant to disease, industry and the environment. In...

Microbial community resemblance methods differ in their ability to detect biologically relevant patterns

Direct sequencing of the human microbiome readily reveals community differences

Culture-independent studies of human microbiota by direct genomic sequencing reveal quite distinct differences among communities, indicating that improved sequencing capacity ca...