Sjors H. W. Scheres

New tools for automated high-resolution cryo-EM structure determination in RELION-3

Here, we describe the third major release of RELION. CPU-based vector acceleration has been added in addition to GPU support, which provides flexibility in use of resources and ...

Accelerated cryo-EM structure determination with parallelisation using GPUs in RELION-2

By reaching near-atomic resolution for a wide range of specimens, single-particle cryo-EM structure determination is transforming structural biology. However, the necessary calc...

A Bayesian View on Cryo-EM Structure Determination

Ribosome structures to near-atomic resolution from thirty thousand cryo-EM particles

Although electron cryo-microscopy (cryo-EM) single-particle analysis has become an important tool for structural biology of large and flexible macro-molecular assemblies, the te...

Prevention of overfitting in cryo-EM structure determination

High-resolution noise substitution to measure overfitting and validate resolution in 3D structure determination by single particle electron cryomicroscopy

Cryo-EM structure of the Plasmodium falciparum 80S ribosome bound to the anti-protozoan drug emetine

Malaria inflicts an enormous burden on global human health. The emergence of parasite resistance to front-line drugs has prompted a renewed focus on the repositioning of clinica...

Single-particle cryo-EM at atomic resolution

Abstract The three-dimensional positions of atoms in protein molecules define their structure and provide mechanistic insights into the roles they perform in complex biological ...